Innovation and novel aspects of the project include:

 

1) The establishment of a strategy, methodologies and technologies for the discovery and production of bioactive enzymes able to cope with the global emergence of multidrug-resistant bacteria.

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2) Exploitation of biological and microbial biodiversity, derived from clinical and aquatic thermophilic ecosystems, through metagenomics analysis with the aim at discovering new and/or thermostable lytic enzymes.

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3) The application of multidisciplinary approaches based on modern -omics technologies and protein engineering in order to design new enzyme variants with purposed-designed catalytic and biochemical properties towards Gram-negative bacteria. Ready-to-deploy portfolios of Enzybiotic variants will be created from biological diverse sources and through protein engineering. 

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4) Structural and biochemical data will provide new information on understanding the genetic, structural, and catalytic mechanisms of native and engineered Enzybiotics.

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5) The implementation of the project will allow the development of additional proposed applications of enzybiotics in several scientific areas such as agricultural, veterinary and for controlling bacterial contamination at different points within the food production chain.